Protocols

Rapid Yeast Transformation

  1. Plate cells 3-4 days at 30ºC
  2. Resuspend a single colony in 70 µL PLATE (TRIS pH 4.9 or 7.5)
  3. Add 10 µL of plasmid DNA (1.0 µg in sterile H2O)
  4. Add 10 µL of carrier DNA (tRNA / 100 µg)
  5. Add 10 µL of sterile H2O (final volume = 100 µL)
  6. Incubate 1h at 30ºC (without agitation)
  7. Heat shock 15 minutes at 42ºC
  8. Cool down to room temperature (wait 2-4 hours to allow antibiotic resistance, if necessary)
  9. Plate
Protocole from : A simple and efficient procedure for transformation of Schizosaccharomyces pombe.
Morita T, Takegawa K.; Yeast. 2004 Jun;21(8):613-7.; PMID: 15197727 [PubMed - in process]